Protection of islet allografts transplanted together with Fas ligand expressing testicular allografts

CTLA4-Fas ligand gene transfer mediated by adenovirus induce long-time survival of murine cardiac allografts.

BACKGROUND Fas ligand gene transfer to induce peripheral allograft tolerance in animal models has shown controversial results. The immunosuppression effects mediated by engineered FasL depend on whether alloreactive T cells are selectively deleted. In the present study, we tested the feasibility of a strategy to induce long-time survival by fusing CTLA4-FasL gene transfer in vivo. METHODS Car...

Significant manipulative procedures to reduce immunogenicity of human islet allografts.

Immunofluorescence studies and mixed Iymphocyte islet culture (MLlC) tests were performed using cryopreserved crude human islet preparations l and various HLAas well as cell type-specific monoclonal antibodies in order to (a) locate the potentially immunogenic cells and (b) demonstrate their functional relevance before and after antibody incubation. Microscopic evaluation of exocrine and endocr...

Bioactivities of Fas ligand-expressing retroviral particles.

Culture supernatants from retroviral packaging cells carrying the human Fas ligand (FasL) gene killed both human (Jurkat) and mouse (LB27.4) targets within 5 h of incubation. Cytotoxicity was found both in a fraction >/=500 kDa and a fraction between 50 and 500 kDa. Following ultracentrifugation, the activity in the >/=500-kDa fraction was concentrated in the pellet (FasL vector preparation (VP...

Testicular Sertoli Cells Protect Islet -Cells From Autoimmune Destruction in NOD Mice by a Transforming Growth Factor- 1–Dependent Mechanism

Testicular Sertoli cells protect pancreatic islet grafts from alloand autoimmune destruction; however, the mechanism(s) of protection is unclear. The aim of this study was to determine whether Fas ligand (FasL) and/ or transforming growth factor (TGF), immunoregulatory proteins produced by Sertoli cells, might mediate the protective effects of these cells against autoimmune destruction of islet...

Effect of intraportal human islet transplantation on kidney graft survival in simultaneous kidney-islet allografts.